Oxygraf

Administrative officer

Charlotta Andersson

Institution / equivalent

Department of Biochemistry and Biophysics (DBB)

Inquiry

About Stockholm University

Stockholm University is characterized by openness and an innovative and cross-border activity in Sweden´s capital. Ranked as one of the world´s top 100 HEIs, Stockholm University is one of Europe´s leading institutions for higher education and research in human sciences and natural sciences.

Stockholm University was founded in 1878 with the ambition to renew the traditional universities in Sweden. Close contacts and an active exchange of experience and knowledge with the outside world characterize the business since its inception.

33,000 students, 1,600 doctoral students and 5,500 employees are active at Stockholm University. Within the science areas of science and human sciences, 190 study programs and 1,700 courses are offered. The university has a total turnover of SEK 4.93 billion.

Stockholm University contributes to knowledge development in a global perspective and in collaboration with others. This is done through education and research in the international front line, as well as in cross-border and multidisciplinary studies. In collaboration with the surrounding society, knowledge is made available to everyone through dialogue and participation in debate and social development.

1 About the contracting institution

The department of Biochemistry and Biophysics (DBB) is mainly located at the Arrhenius laboratory in Frescati, close to the other Chemistry and Life Sciences departments. The department also has a strong connection to the Science for Life Laboratory, where some research groups are also located. More than 200 people, including ~80 PhD students, are currently working at the department. The research at DBB spans a wide range of fields; including cell biology, biochemistry, neurochemistry, biophysics, bioinformatics and computational chemistry. Many of the research projects conducted here are of the highest international class. For more information about our business, see our website www.dbb.su.se.

2 Goals and purpose

The department needs to buy an oxygraph. The instrument will primarily be used for measuring the oxygen consumption of chemical reactions catalyzed by respiratory complexes from various species of Actinobacteria.

The purpose of the project is to study the protein complexes that perform the process of cellular respiration in Actinobacteria. The results are important for our understanding of how these respiratory complexes work and why they differ so much from the equivalent protein complexes in other organisms, as well as for the development of drugs designed to interfere with the respiratory process (the clade of Actinobacteria encompasses several pathogens; including the species causing tuberculosis and lepracy).

We have recently been awarded a grant from the KAW foundation for this project, and need to expand our capacity for oxygen consumption measurements in order to perform the experiments. In the future, the instrument will also be used for similar purposes in other research projects at the department.

3  Requirement specification

Technical requirements

The instrument:

Must be based on a Clark electrode (silver and platinum)

Must have a low background level of oxygen consumption (<0,015 µM/h)

Must work reliably within a wide range of measurement volumes (at least 0.2-2,5 ml)

Must have a glass measurement chamber, with a transparent outer shell

Must be equipped for cooling/heating using an external, thermostat controlled water bath (the water bath itself should not be included in the offer though)

Must be able to measure within a wide pH range (at least pH 0-14)

Must be able to measure oxygen concentrations ≤0,0003%

Other requirements

The offer must include all costs for delivery

The product should be delivered as soon as possible, but must be delivered no later than November 2020.

The instrument must be CE labelled

The warranty period of the instrument must be at least one year

4 Quotation

We expect quotation regarding the above and wish the following to be stated in the quotation:

Price; state the total price for delivery including service and installation. No other costs may be added for the commission´s implementation.

Answer in the tender how the offered product meets the requirement specifications.

The quotation will be submitted no later than 2020-09-10

The quotation must be binding with 2020-12-31

5 Evaluation and award (supplier selection)

The supplier offering the most advantageous solution to the contract is awarded the contract. SU evaluates the tenders in accordance with the above requirements.

The instrument must fulfill the requirements listed in the requirement specifications (3). If more than one quotation fulfills all of those requirements, the contract shall be awarded to the supplier offering the lowest price.