Metabolomics

Analysis of frozen blood samples for plasma metabolites. Analysis must allow annotation of at least 1000 metabolites must be analyzed in an EDTA plasma volume of 100-200 microliters. Initially a total of 4000-5000 samples will be analyzed, of which 1200 samples relates to purchases by Prof David Erlinge, IKVL, and the remaining Gunnar Engström and Gustav Smith.

Delivery of initial 4000- 5000 analysis by the end of 2019 or according to agreement with the buyer.

Contract will be signed with one supplier.

Estimated contract period 2019-09-01 -- 2020-08-31 with possible extension until 2021-08-31

The procurement covers non-targeted mass spectrometry-based metabolomics analysis with relative or absolute quantification of metabolites in plasma from clinical and population-based studies at Department of Clinical Sciences in Malmö and Lund, Lund University (the SCAPIS study in Malmö “SCAPIS” (n=3,000 samples), the “PROSPECT2”-project (n=900) and the PROFLOW study (n=300) .

Scope

Analysis of frozen blood samples for plasma metabolites. Analysis must allow annotation of at least 1000 metabolites must be analyzed in an EDTA plasma volume of 100-200 microliters. Initially a total of 4000-5000 samples will be analyzed, of which 1200 samples relates to purchases by Prof David Erlinge, IKVL, and the remaining Gunnar Engström and Gustav Smith.

Additional analysis may be needed depending on the developement of the study.

Method: LC-MS

Quality measure: Majority of analysis <15% variability

Access to current library of plasma metabolites.

Delivery of initial 4000- 5000 analysis by the end of 2019 or according to agreement with the buyer.

Contract will be signed with one supplier.

Estimated contract period 2019-09-01 -- 2020-08-31 with possible extension until 2021-08-31

The procurement covers non-targeted mass spectrometry-based metabolomics analysis with relative or absolute quantification of metabolites in plasma from clinical and population-based studies at Department of Clinical Sciences in Malmö and Lund, Lund University (the SCAPIS study in Malmö “SCAPIS” (n=3,000 samples), the “PROSPECT2”-project (n=900) and the PROFLOW study (n=300) . The assignment includes:1. Sample receipt and registration2. Non-targeted mass spectrometry-based metabolomics analysis in human plasma.3. Delivery of results to customer4. Destruction of remaining samples or return of samples to the university (will be specified later).

Additional samples might need to be analysed. Therefore, the procurement also includes an option for the University to extend the contract for analysis of addtitional samples.

It is crucial that the performed analysis is of excellent quality and that the results can used for scientific comparison with other ongoing studies like SCAPIS in Uppsala.