Kungliga Tekniska Högskolan, KTH Procurement of 10X Genomics Visium kits

Procurement of 10X Genomics Visium kits

Kungliga Tekniska högskolan, STOCKHOLM

Part of NGI Stockholms mission as a state-of-the-art facility is making novel techniques available to the research community. Due to this, NGI is now planning to start running the Spatial Transcriptomics method which enables spatially resolved RNA expression data to be collected from tissue sections. This method uses kits sold under the brand name Visium to be purchased from 10x Genomics as they currently are the only provider of these kits.

Part of NGI Stockholms mission as a state-of-the-art facility is making novel techniques available to the research community. Due to this, NGI is now planning to start running the Spatial Transcriptomics, enabling global assessment of RNA expression in a spatially resolved manner along the area of a tissue section. Briefly, RNA molecules expressed at thousands of spatially defined coordinates in the tissue are subject to cDNA syntesis using oligonucleotides carrying coordinate specific sequence motifs. It is known which sequence motifs correspond to which coordinate and this enables spatially resolved RNA expression levels to be determined after sequencing the synthesized cDNA. This method uses kits sold under the brand name “Visium Spatial” to be purchased from 10x Genomics as they currently are the only provider of a solution enabling transciptome-wide, non-targeted Spatial Transciptomics.

(Meddelande om frivillig förhandsinsyn)

Förfarande

Ej angiven

Publicerad

2019-09-13

Dokumenttyp

Meddelande om frivillig förhandsinsyn

Leveransorter

Stockholms län

Diarie-/referensnummer

C-2019-1688